Photo inducible RNA interference using cell permeable protein carrier (2007)
Sequence: HIV-1 Tat peptide fused to UIA RBD
| Experiment Id | EXP002122 |
|---|---|
| Paper | Photo inducible RNA interference using cell permeable protein carrier |
| Peptide | Tat-UIA |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 2 µM |
| Rna Concentration | 200 nM |
| Mixing Ratio | TatUIA 2 µM : RNA 200 nM |
| Formulation Format | CPP–protein carrier complex |
| Formulation Components | Tat peptide + UIA RNA-binding domain protein |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown (photo-inducible) |
| Output Value | Efficient EGFP silencing only within irradiated region |
| Output Units | |
| Output Notes | RNA delivery and endosomal escape triggered by light; no in vivo experiments |
| Toxicity Notes | Not reported; proof-of-concept study |
| Curation Notes |