Use of cell cultures in vitro to assess the uptake of long dsRNA in plant cells (2022)
Sequence: (Tat)x3-HA-PKR dsRNA-binding fusion protein
| Experiment Id | EXP002123 |
|---|---|
| Paper | Use of cell cultures in vitro to assess the uptake of long dsRNA in plant cells |
| Peptide | cCPP (3×Tat-HA-PKR) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~935 pmol (carrier) |
| Rna Concentration | ~1.87 pmol dsRNA |
| Mixing Ratio | 500:1 (carrier:dsRNA) |
| Formulation Format | CPP–protein carrier complex |
| Formulation Components | Tat-based CPP + HA domain + PKR dsRNA-binding domain |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Arabidopsis thaliana PSB-D; Nicotiana tabacum BY-2 cells |
| Animal Model | |
| Administration Route | |
| Output Type | uptake / internalization |
| Output Value | Cy5-dsRNA internalized in PSB-D cells; not in BY-2 cells |
| Output Units | |
| Output Notes | Confocal microscopy shows accelerated uptake with cCPP; no RNAi or gene silencing measured |
| Toxicity Notes | No toxicity observed during uptake experiments |
| Curation Notes |