Effect of small molecule signaling in PepFect14 transfection (2020)
Sequence: Stearyl-AGYLLGKLLOOLAAAAALOOLL-NH2
splice-correcting oligonucleotide
| Experiment Id | EXP002124 |
|---|---|
| Paper | Effect of small molecule signaling in PepFect14 transfection |
| Peptide | PepFect14 (PF14) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 2 µM |
| Rna Concentration | 0.4 µM |
| Mixing Ratio | PF14:SCO 5:1 (mol/mol) |
| Formulation Format | CPP–oligonucleotide nanocomplex |
| Formulation Components | PepFect14 + SCO |
| Size Nm | 300.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 cells |
| Animal Model | |
| Administration Route | |
| Output Type | splice correction / luciferase expression |
| Output Value | Up to ~100-fold increase in luciferase signal (enhanced with small molecules) |
| Output Units | |
| Output Notes | Functional SCO delivery in vitro; enhanced uptake via endocytosis modulation; no in vivo studies |
| Toxicity Notes | Low toxicity for PF14:SCO alone; drug-dependent effects |
| Curation Notes |