Back to browse

EXP002125

Paper

Supplementation With Cell-Penetrating Peptide-Conjugated Estrogen-Related Receptor β Improves the Formation of the Inner Cell Mass and the Development of Vitrified/Warmed Mouse Embryos (2016)

Peptide

CPP-ESRRB (7R-ESRRB)

Sequence: RRRRRRR-ESRRB

RNA

mRNA (endogenous regulation)

All experiment fields

Experiment Id EXP002125
Paper Supplementation With Cell-Penetrating Peptide-Conjugated Estrogen-Related Receptor β Improves the Fo
Peptide CPP-ESRRB (7R-ESRRB)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration 2 mg/mL
Rna Concentration
Mixing Ratio protein added to culture medium
Formulation Format CPP–protein delivery
Formulation Components 7R CPP + ESRRB recombinant protein
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Mouse embryos (8-cell to blastocyst)
Animal Model
Administration Route
Output Type gene upregulation / ICM formation
Output Value Increased Oct4 and Nanog expression; increased ICM cell number
Output Units
Output Notes CPP-mediated protein delivery into embryos improved pluripotency markers
Toxicity Notes No developmental toxicity; healthy pups born
Curation Notes