Sequence: GLFEAIEGFIENGWEGMIDGWYGGGGrrarrrrrrK
| Experiment Id | EXP002127 |
|---|---|
| Paper | Development of a cell-penetrating peptide-based nanocomplex for long-term delivery of intact mitocho |
| Peptide | RD3AD |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | varied (N:P 11.25 optimal) |
| Rna Concentration | 500 ng–2 µg mtDNA |
| Mixing Ratio | N:P 11.25 |
| Formulation Format | CPP–DNA nanocomplex |
| Formulation Components | RD3AD + mtDNA + Rhodamine 123 |
| Size Nm | 150.00 |
| Zeta Mv | 3.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | ARPE-19; mtDNA-depleted ρ0 cells |
| Animal Model | |
| Administration Route | |
| Output Type | mtDNA retention; mitochondrial RNA/protein expression |
| Output Value | ≥4 weeks retention; ↑ mtRNR2, MT-CO2 |
| Output Units | |
| Output Notes | Functional mitochondrial transcription and translation restored |
| Toxicity Notes | No cytotoxicity (TEER, MTS) |
| Curation Notes |