Synthesis of cell penetrating peptide sterol coupler and its liposome study on S-mRNA (2023)
Sequence: CGYKK
| Experiment Id | EXP002152 |
|---|---|
| Paper | Synthesis of cell penetrating peptide sterol coupler and its liposome study on S-mRNA |
| Peptide | chol-CGYKK (Liposome 3) |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 5 µg mRNA per mouse (per immunization group); LNP 5 µg as control |
| Mixing Ratio | liposome:mRNA = 10:1 (w/w); gel retardation complete at 8:1; used 10:1 thereafter |
| Formulation Format | CPP-modified cationic liposome |
| Formulation Components | DOTAP:DOPE:DPPC:chol-CGYKK:MSPC = 3.5:1:1:1.5:3 (molar) |
| Size Nm | 135.70 |
| Zeta Mv | 40.86 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | BALB/c mice (female, 6–8 weeks) |
| Administration Route | intramuscular (day 0, day 14) |
| Output Type | Spike-specific IgG titer (ELISA) |
| Output Value | |
| Output Units | |
| Output Notes | IgG response detected but weak; authors report no significant differences among the four liposome-formulated samples and overall inferior to LNP control; liposome group did not improve from day 14 to day 28. |
| Toxicity Notes | No toxic effects reported in safety evaluation of CGYKK and vaccine liposomes. |
| Curation Notes |