Sequence: K16GACSERSMNFCG
| Experiment Id | EXP002165 |
|---|---|
| Paper | Low Molecular Weight Alginate Oligosaccharides as Alternatives to PEG for Enhancement of the Diffusi |
| Peptide | Peptide E |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 ng per well (in vitro) |
| Mixing Ratio | liposome:peptide:mRNA = 1:4:1 (w/w) |
| Formulation Format | Receptor-targeted nanocomplex (RTN) ± alginate/PEG |
| Formulation Components | DOTMA/DOPE liposome + Peptide E + mRNA ± OligoG/OligoM/PEG |
| Size Nm | 120.00 |
| Zeta Mv | 30.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CFBE BMI-1; NHBE BMI-1 |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter expression (luciferase) |
| Output Value | Robust expression; OligoM ≈ cationic RTN |
| Output Units | |
| Output Notes | Functional mRNA delivery demonstrated in vitro; study focuses on mucus diffusion and formulation effects. |
| Toxicity Notes | Low toxicity; alginates reduce cytotoxicity |
| Curation Notes |