A Novel Cell-Penetrating Peptide–VEGF siRNA Complex That Mediates Inhibition of Angiogenesis (2025)
Sequence: RNQRKTVRCFRCRQAGHWISDCRLKSK
| Experiment Id | EXP002185 |
|---|---|
| Paper | A Novel Cell-Penetrating Peptide–VEGF siRNA Complex That Mediates Inhibition of Angiogenesis |
| Peptide | Ara27 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1 µM |
| Rna Concentration | 50 nM |
| Mixing Ratio | 1:20 (optimal) |
| Formulation Format | CPP/siRNA complex |
| Formulation Components | Ara27 + siVEGF |
| Size Nm | 180.00 |
| Zeta Mv | |
| Model Scope | ex_vivo |
| Model Type | in vitro + ex vivo |
| Cell Lines Or Primary Cells | HUVECs |
| Animal Model | Ex vivo mouse aorta ring |
| Administration Route | |
| Output Type | VEGF knockdown; angiogenesis inhibition |
| Output Value | ≈70% VEGF mRNA reduction |
| Output Units | |
| Output Notes | Reduced VEGF signaling, migration, tube formation, and aortic sprouting |
| Toxicity Notes | Low cytotoxicity and no immunotoxicity |
| Curation Notes |