Sequence: VQWRIRVAVIRRVVVSR + C12 fatty acid
| Experiment Id | EXP002186 |
|---|---|
| Paper | In silico optimized cell-penetrating peptides achieve transdermal siRNA delivery and regulate inflam |
| Peptide | LAU-DP7 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | variable (gel retardation optimized) |
| Rna Concentration | 5 µg/mL (Cy5-siRNA) |
| Mixing Ratio | 3:1 (peptide:siRNA, w/w) |
| Formulation Format | FA-DP7/siRNA@Gelatin spray |
| Formulation Components | FA-DP7 + siRNA + gelatin + NHS-PEG-MAL |
| Size Nm | 80.00 |
| Zeta Mv | 8.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HaCaT; dendritic cells; 3D T-SKIN™ |
| Animal Model | |
| Administration Route | |
| Output Type | siRNA uptake, lysosomal escape, gene knockdown |
| Output Value | PAL/STE > MYR > LAU |
| Output Units | |
| Output Notes | Functional ADAM17 knockdown, cytokine suppression, lysosomal escape shown |
| Toxicity Notes | Low cytotoxicity below 20 µg/mL |
| Curation Notes |