Sequence: ACTGSTQHQCG
| Experiment Id | EXP002253 |
|---|---|
| Paper | Transdermal siRNA delivery by pH-switchable micelles with targeting effect suppress skin melanoma pr |
| Peptide | SCP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | SHP:siRNA w/w 2:1, 5:1, 10:1; N/P not specified for transfection assay |
| Formulation Format | pH-switchable cationic micelles (SHP) assembled from SCP-HA-PAE |
| Formulation Components | SCP (ACTGSTQHQCG) + hyaluronic acid (HA, MW 8000) + poly(β-amino ester) (PAE) |
| Size Nm | 170.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HCT116-Luc |
| Animal Model | |
| Administration Route | cell culture (transfection; 6 h exposure; micelles 1 μg/mL) |
| Output Type | reporter knockdown (luciferase assay) |
| Output Value | up to ~62% knockdown at SHP:siRNA = 5:1 (w/w) |
| Output Units | |
| Output Notes | siRNA-luciferase delivered by SHP; luciferase activity normalized by total protein. |
| Toxicity Notes | SHP showed mild cytotoxicity in B16F10 (≈83% viability at 10 μg/mL). |
| Curation Notes |