Sequence: ACTGSTQHQCG
| Experiment Id | EXP002254 |
|---|---|
| Paper | Transdermal siRNA delivery by pH-switchable micelles with targeting effect suppress skin melanoma pr |
| Peptide | SCP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | SHP/siRNA N/P = 10:1 (used for release & uptake studies); survivin-siRNA loaded SHP |
| Formulation Format | pH-switchable cationic micelles (SHP) assembled from SCP-HA-PAE |
| Formulation Components | SCP (ACTGSTQHQCG) + hyaluronic acid (HA, MW 8000) + poly(β-amino ester) (PAE) |
| Size Nm | 170.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | B16F10 (murine melanoma) |
| Animal Model | |
| Administration Route | cell culture |
| Output Type | apoptosis induction (Annexin V/PI; survivin slicing implied) |
| Output Value | ~4-fold higher apoptosis vs naked siRNA or blank micelles (24 h) |
| Output Units | |
| Output Notes | Also showed cellular uptake (FCM/CLSM) and lysosome escape (LysoTracker signal decreases; endosome burst). |
| Toxicity Notes | Blank SHP: ~83% viability retained at 10 μg/mL; low toxicity attributed to degradable components. |
| Curation Notes |