Potent Protein Delivery into Mammalian Cells via a Supercharged Polypeptide (2018)
Sequence: supercharged polypeptide (~240 aa; K-rich; exact sequence not disclosed)
| Experiment Id | EXP002256 |
|---|---|
| Paper | Potent Protein Delivery into Mammalian Cells via a Supercharged Polypeptide |
| Peptide | SCP (K4) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ~2 µM (protein fusion) |
| Rna Concentration | |
| Mixing Ratio | genetic fusion (1:1) |
| Formulation Format | protein–polypeptide fusion |
| Formulation Components | SCP(K4)-GFP |
| Size Nm | 9.40 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa, A549, BEL-7402, PC12, C6, Jurkat |
| Animal Model | |
| Administration Route | cell incubation (2 h, 37 °C) |
| Output Type | cellular uptake + nuclear localization |
| Output Value | high uptake; predominant nuclear accumulation |
| Output Units | |
| Output Notes | Flow cytometry and confocal microscopy; heparin wash removes surface-bound protein. |
| Toxicity Notes | No significant cytotoxicity up to 10 µM; minimal hemolysis. |
| Curation Notes |