Potent Protein Delivery into Mammalian Cells via a Supercharged Polypeptide (2018)
Sequence: supercharged polypeptide (~240 aa; K-rich; exact sequence not disclosed)
| Experiment Id | EXP002257 |
|---|---|
| Paper | Potent Protein Delivery into Mammalian Cells via a Supercharged Polypeptide |
| Peptide | SCP (K4) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | Cas9-K4 protein complex |
| Rna Concentration | |
| Mixing Ratio | Cas9-K4:sgRNA ≥4:1 (w/w) |
| Formulation Format | protein–polypeptide fusion + RNP complex |
| Formulation Components | Cas9-K4 + sgRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa, HEK293T, Jurkat |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | genome editing (indel formation) |
| Output Value | up to ~15.2% indels at CCR5 locus |
| Output Units | |
| Output Notes | T7 endonuclease I assay; no editing at 4 °C or without sgRNA. |
| Toxicity Notes | Low cytotoxicity; no innate cytokine induction. |
| Curation Notes |