Sequence: RKKRRQRRRR + 10×His + 2×Cys (C-5H-Tat-5H-C)
| Experiment Id | EXP002258 |
|---|---|
| Paper | Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified |
| Peptide | mTAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | variable; optimal mTAT:PEI:DNA = 8:8:1 (w/w) |
| Rna Concentration | 2 µg plasmid per well |
| Mixing Ratio | mTAT:PEI:DNA = 8:8:1 (w/w) |
| Formulation Format | polyplex |
| Formulation Components | mTAT + PEI + plasmid DNA |
| Size Nm | 180.00 |
| Zeta Mv | 30.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | hUCB-MSC |
| Animal Model | |
| Administration Route | cell transfection (4–6 h incubation) |
| Output Type | gene expression (GFP) |
| Output Value | highest GFP-positive fraction vs PEI25 or PEI |
| Output Units | |
| Output Notes | Flow cytometry and fluorescence microscopy show enhanced GFP expression. |
| Toxicity Notes | mTAT/PEI showed significantly lower toxicity than PEI25 (MTT assay). |
| Curation Notes |