Sequence: RKKRRQRRRR + 10×His + 2×Cys (C-5H-Tat-5H-C)
| Experiment Id | EXP002259 |
|---|---|
| Paper | Modification of Human Umbilical Cord Blood Stem Cells Using Polyethylenimine Combined with Modified |
| Peptide | mTAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | mTAT:PEI optimized |
| Rna Concentration | 2 µg gWiz-BMP2 plasmid |
| Mixing Ratio | mTAT:PEI:DNA = 8:8:1 (w/w) |
| Formulation Format | polyplex |
| Formulation Components | mTAT + PEI + BMP-2 plasmid |
| Size Nm | 180.00 |
| Zeta Mv | 30.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | hUCB-MSC |
| Animal Model | |
| Administration Route | cell transfection |
| Output Type | BMP-2 expression + osteogenic differentiation |
| Output Value | ↑ BMP-2 mRNA/protein; ↑ ALP activity; ↑ calcium deposition |
| Output Units | |
| Output Notes | ELISA, RT-PCR, Western blot (p-Smad1/5/8), ALP assay, Alizarin Red staining. |
| Toxicity Notes | Minimal toxicity compared to PEI25; sustained viability up to 72 h. |
| Curation Notes |