Sequence: CKRRMKWKK
| Experiment Id | EXP002260 |
|---|---|
| Paper | Sequential delivery of VEGF siRNA and paclitaxel for PVN destruction, anti-angiogenesis, and tumor c |
| Peptide | CPP (penetratin-derived) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | siVEGF-CPPs ~100 nM (functional assays) |
| Rna Concentration | siVEGF 100 nM |
| Mixing Ratio | siVEGF:CPP 1:1 (disulfide conjugate) |
| Formulation Format | polymeric micelle (TMPM) |
| Formulation Components | Folate-PEG-PHIS + PCL-PEG-PHIS + siVEGF-CPPs ± PTX |
| Size Nm | 100.00 |
| Zeta Mv | 5.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 / HUVEC co-culture |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | VEGF gene silencing + anti-proliferation |
| Output Value | ↓ VEGF mRNA/protein; ↑ apoptosis (synergy with PTX) |
| Output Units | |
| Output Notes | qRT-PCR, ELISA, CLSM, LysoTracker shows endosomal escape at 2 h. |
| Toxicity Notes | Low toxicity at siRNA-relevant doses |
| Curation Notes |