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EXP002266

Paper

NickFect type of cell-penetrating peptides present enhanced efficiency for microRNA-146a delivery into dendritic cells and during skin inflammation (2020)

Peptide

NF71

Sequence: Stearyl-HHYHHGOaILLKALKALAKAIL-NH2

RNA

miRNA mimic

All experiment fields

Experiment Id EXP002266
Paper NickFect type of cell-penetrating peptides present enhanced efficiency for microRNA-146a delivery in
Peptide NF71
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration CPP:miRNA MR 17:1–28:1
Rna Concentration 30–100 nM
Mixing Ratio CPP:miRNA 17:1 or 28:1
Formulation Format CPP–miRNA nanocomplex
Formulation Components NF71 + miR-146a
Size Nm 15.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells primary keratinocytes; monocyte-derived dendritic cells
Animal Model
Administration Route cell incubation
Output Type target gene suppression
Output Value ↓ IRAK1, CARD10, IL-6, CXCL8 (CPP-dependent)
Output Units
Output Notes NF-type CPPs outperform PF/MGPE in DCs
Toxicity Notes no cytotoxicity; no DC activation
Curation Notes