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EXP002270

Paper

NickFect type of cell-penetrating peptides present enhanced efficiency for microRNA-146a delivery into dendritic cells and during skin inflammation (2020)

Peptide

NF71

Sequence: Stearyl-HHYHHGOaILLKALKALAKAIL-NH2

RNA

miRNA mimic

All experiment fields

Experiment Id EXP002270
Paper NickFect type of cell-penetrating peptides present enhanced efficiency for microRNA-146a delivery in
Peptide NF71
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration 60 pmol per ear
Rna Concentration 60 pmol per ear
Mixing Ratio CPP:miRNA 28:1
Formulation Format CPP–miRNA nanocomplex
Formulation Components NF71 + miR-146a
Size Nm 50.00
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model C57BL/6 mouse ICD model
Administration Route subcutaneous ear injection
Output Type inflammation suppression
Output Value ↓ ear swelling; ↓ IL-6, IL-1β, IL-33, TNF-α
Output Units
Output Notes functional miRNA delivery in vivo
Toxicity Notes no inflammation without PMA
Curation Notes