Cell-Selective Delivery of MicroRNA with a MicroRNA–Peptide Conjugate Nanocomplex (2018)
Sequence: FA-GGG-RRRRRRRRR
| Experiment Id | EXP002272 |
|---|---|
| Paper | Cell-Selective Delivery of MicroRNA with a MicroRNA–Peptide Conjugate Nanocomplex |
| Peptide | FA-R9 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | peptide:miRNA 50:1 (molar) |
| Rna Concentration | 100 nM |
| Mixing Ratio | 50:1 (peptide:miRNA) |
| Formulation Format | miRNA nanocomplex (MIRNC) |
| Formulation Components | FA-R9 + miR-34a |
| Size Nm | 50.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87MG, HeLa (co-culture and monoculture) |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | miRNA delivery and target gene repression |
| Output Value | ↑ intracellular miR-34a; ↓ luciferase reporter activity |
| Output Units | |
| Output Notes | RT-qPCR and reporter assays confirm functional miR-34a delivery; cell-selective uptake via receptor-mediated endocytosis. |
| Toxicity Notes | No cytotoxicity reported |
| Curation Notes |