Sequence: VSRRRRRRGGRRRR
| Experiment Id | EXP002273 |
|---|---|
| Paper | Control of cancer stem cell like population by intracellular target identification followed by the t |
| Peptide | LMWP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | siRNA:LMWP = 1:10 (charge ratio) |
| Rna Concentration | 80 nM |
| Mixing Ratio | 1:10 (siRNA:LMWP) |
| Formulation Format | CPP–siRNA complex |
| Formulation Components | LMWP + FER siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231 CD44High breast cancer stem-like cells |
| Animal Model | |
| Administration Route | cell transfection |
| Output Type | gene knockdown and phenotypic suppression |
| Output Value | ↓ FER protein; ↓ migration; ↓ sphere formation; ↓ EMT markers |
| Output Units | |
| Output Notes | Western blot, RT-qPCR, scratch assay, sphere-formation assay confirm functional FER silencing. |
| Toxicity Notes | LMWP shows low cytotoxicity compared to Lipofectamine |
| Curation Notes |