Delivery of 2-5A cargo into living cells using the Tat cell penetrating peptide: 2-5A-tat (2006)
Sequence: RKKRRQRRR
| Experiment Id | EXP002274 |
|---|---|
| Paper | Delivery of 2-5A cargo into living cells using the Tat cell penetrating peptide: 2-5A-tat |
| Peptide | Tat(48–57) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 125–500 nM |
| Rna Concentration | equimolar (conjugated) |
| Mixing Ratio | covalent 1:1 |
| Formulation Format | CPP–oligonucleotide conjugate |
| Formulation Components | Tat peptide + 2-5A tetramer |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells (RNase L overexpression) |
| Animal Model | |
| Administration Route | direct incubation |
| Output Type | RNA degradation |
| Output Value | 28S/18S rRNA cleavage via RNase L |
| Output Units | |
| Output Notes | Functional intracellular RNase L activation demonstrated without transfection reagent. |
| Toxicity Notes | Cell rounding at high concentration; no systemic toxicity assessed |
| Curation Notes |