Comparison of Nanomaterials for Delivery of Double-Stranded RNA in Caenorhabditis elegans (2020)
Sequence: (Lys)n polymer
| Experiment Id | EXP002276 |
|---|---|
| Paper | Comparison of Nanomaterials for Delivery of Double-Stranded RNA in Caenorhabditis elegans |
| Peptide | poly-L-lysine (PLK) |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | no |
| Peptide Concentration | 5:1 polymer:dsRNA (mass) |
| Rna Concentration | 50–400 ng/µL |
| Mixing Ratio | 5:1 (mass) |
| Formulation Format | polyplex |
| Formulation Components | poly-L-lysine + dsRNA |
| Size Nm | 350.00 |
| Zeta Mv | 35.00 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Caenorhabditis elegans |
| Administration Route | oral soaking |
| Output Type | gene knockdown |
| Output Value | no knockdown |
| Output Units | |
| Output Notes | limited uptake; dsRNA retained in pharynx |
| Toxicity Notes | no major toxicity |
| Curation Notes |