Peptide generated anisotropic gold nanoparticles as efficient siRNA vectors (2019)
Sequence: WRWRWR
| Experiment Id | EXP002281 |
|---|---|
| Paper | Peptide generated anisotropic gold nanoparticles as efficient siRNA vectors |
| Peptide | LP5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Au:peptide 1:1 (nanoparticle synthesis) |
| Rna Concentration | 100 pmol per well |
| Mixing Ratio | LP-GNP:siRNA 1:40–1:60 |
| Formulation Format | peptide-derived gold nanoparticle (LP-GNP) |
| Formulation Components | LP5-GNP + siRNA |
| Size Nm | 20.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HCT116 colon cancer cells |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | gene knockdown |
| Output Value | ↓ GAPDH protein and mRNA |
| Output Units | |
| Output Notes | LP4, LP5, LP7, LP8 show strongest knockdown; LP7 serum-stable |
| Toxicity Notes | >90% viability at 5–10 µM |
| Curation Notes |