Cell Penetrating Peptide-Based Self-Assembly for PD-L1 Targeted Tumor Regression (2021)
Sequence: FFAARTMIWY(d-P)GAWYKRI
| Experiment Id | EXP002285 |
|---|---|
| Paper | Cell Penetrating Peptide-Based Self-Assembly for PD-L1 Targeted Tumor Regression |
| Peptide | PDL1-CPP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | ≤15 µM |
| Rna Concentration | 5 µM |
| Mixing Ratio | electrostatic self-assembly |
| Formulation Format | pure peptide nanosphere |
| Formulation Components | peptide + siRNA (± DOX) |
| Size Nm | 170.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231, HUVEC |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | gene knockdown and apoptosis |
| Output Value | ↓ SURVIVIN mRNA/protein; ↑ apoptosis |
| Output Units | |
| Output Notes | qRT-PCR, Western blot, flow cytometry apoptosis assays |
| Toxicity Notes | low cytotoxicity <15 µM |
| Curation Notes |