Sequence: GRKKRRQRRR
| Experiment Id | EXP002291 |
|---|---|
| Paper | Cell-penetrating peptide conjugates of peptide nucleic acids as inhibitors of HIV-1 Tat-dependent tr |
| Peptide | Tat |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 0.3–2.5 µM |
| Rna Concentration | equimolar (conjugated) |
| Mixing Ratio | covalent conjugate |
| Formulation Format | CPP–PNA conjugate |
| Formulation Components | CPP + TAR-targeting PNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa Tet-Off/Tat luciferase reporter cells |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | Tat-dependent transcription inhibition |
| Output Value | dose-dependent luciferase reduction |
| Output Units | |
| Output Notes | Functional inhibition observed; activity enhanced by chloroquine, indicating endosomal escape limitation. |
| Toxicity Notes | low toxicity at tested doses |
| Curation Notes |