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EXP002292

Paper

Cell-penetrating peptide conjugates of peptide nucleic acids as inhibitors of HIV-1 Tat-dependent trans-activation in cells (2005)

Peptide

Transportan

Sequence: GWTLNSAGYLLGKINLKALAALAKKIL

RNA

PNA (antisense analogue)

All experiment fields

Experiment Id EXP002292
Paper Cell-penetrating peptide conjugates of peptide nucleic acids as inhibitors of HIV-1 Tat-dependent tr
Peptide Transportan
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration 0.3–2.5 µM
Rna Concentration equimolar (conjugated)
Mixing Ratio covalent conjugate
Formulation Format CPP–PNA conjugate
Formulation Components CPP + TAR-targeting PNA
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HeLa Tet-Off/Tat luciferase reporter cells
Animal Model
Administration Route cell incubation
Output Type Tat-dependent transcription inhibition
Output Value dose-dependent luciferase reduction
Output Units
Output Notes Strong uptake and functional inhibition; chloroquine enhances activity, indicating partial endosomal trapping.
Toxicity Notes low toxicity at tested doses
Curation Notes