Sequence: RKKRRQRRR
| Experiment Id | EXP002294 |
|---|---|
| Paper | Synthesis of TAT peptide-tagged PEGylated chitosan nanoparticles for siRNA delivery targeting neurod |
| Peptide | TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | polymer 0.5 mg/mL |
| Rna Concentration | 40 µM (cell experiments) |
| Mixing Ratio | electrostatic complexation with TPP |
| Formulation Format | PEGylated chitosan nanoparticle (peptide-tagged) |
| Formulation Components | chitosan-PEG-TAT + siRNA |
| Size Nm | 5.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Neuro2a mouse neuroblastoma cells |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | gene knockdown |
| Output Value | ↓ Ataxin-1 protein after 48 h |
| Output Units | |
| Output Notes | Western blot; efficient transfection with reduced toxicity vs chitosan |
| Toxicity Notes | minimal toxicity compared to unmodified chitosan |
| Curation Notes |