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EXP002303

Paper

Reshaping Prostate Tumor Microenvironment To Suppress Metastasis via Cancer-Associated Fibroblast Inactivation with Peptide-Assembly-Based Nanosystem (2019)

Peptide

Chol-R9

Sequence: (cholesterol)2-Lys-(Gly)2-(Arg)9

RNA

siRNA

All experiment fields

Experiment Id EXP002303
Paper Reshaping Prostate Tumor Microenvironment To Suppress Metastasis via Cancer-Associated Fibroblast In
Peptide Chol-R9
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration 25–100 µg/mL (PNP)
Rna Concentration 100 nM
Mixing Ratio electrostatic assembly
Formulation Format peptide nanoparticle
Formulation Components Chol-R9 PNP + siRNA + anti-FAPα mAb
Size Nm 85.00
Zeta Mv -13.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells CAFs (primary)
Animal Model
Administration Route cell culture
Output Type gene silencing
Output Value ~89% CXCL12 knockdown
Output Units
Output Notes qRT-PCR, Western blot, ELISA
Toxicity Notes No cytotoxicity observed
Curation Notes