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EXP002305

Paper

Prolongation of Life in Rats with Malignant Glioma by Intranasal siRNA/Drug Codelivery to the Brain with Cell-Penetrating Peptide-Modified Micelles (2014)

Peptide

Tat-modified MPEG-PCL

Sequence: GRKKRRQRRRCG

RNA

siRNA

All experiment fields

Experiment Id EXP002305
Paper Prolongation of Life in Rats with Malignant Glioma by Intranasal siRNA/Drug Codelivery to the Brain
Peptide Tat-modified MPEG-PCL
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration polymer-based
Rna Concentration 1–10 µg/mL siRNA
Mixing Ratio N/P 30
Formulation Format polymeric micelle
Formulation Components MPEG-PCL-Tat + CPT
Size Nm 60.00
Zeta Mv 13.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells C6 rat glioma cells
Animal Model
Administration Route cell culture
Output Type gene silencing / cytotoxicity
Output Value Raf-1 knockdown; increased cell death
Output Units
Output Notes WST-8 assay; dose-dependent effect
Toxicity Notes No toxicity in neuron cells
Curation Notes