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EXP002307

Paper

Tumor Microenvironment-Responsive Multistaged Nanoplatform for Systemic RNAi and Cancer Therapy (2017)

Peptide

TCPA2

Sequence: C17H35CONH-(C17H35CONH)-KR8GRGDS-OH

RNA

siRNA

All experiment fields

Experiment Id EXP002307
Paper Tumor Microenvironment-Responsive Multistaged Nanoplatform for Systemic RNAi and Cancer Therapy
Peptide TCPA2
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration 10–20 nM siRNA
Rna Concentration 10–20 nM
Mixing Ratio electrostatic complexation
Formulation Format polymeric nanoparticle (multistaged)
Formulation Components Meo-PEG-b-PHMEMA + TCPA2 + siRNA
Size Nm 70.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Luc-HeLa; LNCaP
Animal Model
Administration Route cell culture
Output Type gene silencing
Output Value ~90% Luc knockdown; strong BRD4 silencing
Output Units
Output Notes pH 6.8 enhanced cytosolic distribution
Toxicity Notes Low cytotoxicity
Curation Notes