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EXP002325

Paper

siRNA and pharmacological inhibition of endocytic pathways to characterize the differential role of macropinocytosis and the actin cytoskeleton on cellular uptake of dextran and cationic cell penetrating peptides octaarginine (R8) and HIV-Tat (2012)

Peptide

R8

Sequence: RRRRRRRRGC

RNA

All experiment fields

Experiment Id EXP002325
Paper siRNA and pharmacological inhibition of endocytic pathways to characterize the differential role of
Peptide R8
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration 2 µM (uptake assay)
Rna Concentration
Mixing Ratio
Formulation Format fluorescent peptide probe
Formulation Components Alexa488-R8 (uptake mechanism study; not an RNA carrier)
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HeLa; A431
Animal Model
Administration Route cell culture
Output Type uptake mechanism assay
Output Value Flow cytometry uptake vs time; inhibition by Pak-1 depletion/actin disruption (context-dependent)
Output Units
Output Notes Study uses siRNA knockdown of endocytic proteins to probe uptake routes; no RNA delivery by peptide
Toxicity Notes
Curation Notes