Sequence: GRKKRRQRRRPPQGC
| Experiment Id | EXP002326 |
|---|---|
| Paper | siRNA and pharmacological inhibition of endocytic pathways to characterize the differential role of |
| Peptide | HIV-Tat |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 2 µM (uptake assay) |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | fluorescent peptide probe |
| Formulation Components | Alexa488-Tat (uptake mechanism study; not an RNA carrier) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; A431 |
| Animal Model | |
| Administration Route | cell culture |
| Output Type | uptake mechanism assay |
| Output Value | Flow cytometry uptake vs time; inhibition by Pak-1 depletion/actin disruption (context-dependent) |
| Output Units | |
| Output Notes | Study uses siRNA knockdown of endocytic proteins to probe uptake routes; no RNA delivery by peptide |
| Toxicity Notes | |
| Curation Notes |