Graphene oxide nanosheets in complex with cell penetrating peptides for oligonucleotides delivery (2017)
Sequence: Stearyl-FLKLLKKFLFLKLLKKFL-amide
| Experiment Id | EXP002332 |
|---|---|
| Paper | Graphene oxide nanosheets in complex with cell penetrating peptides for oligonucleotides delivery |
| Peptide | PF221 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1 µM |
| Rna Concentration | 25–100 nM |
| Mixing Ratio | CPP:RNA 10–20 |
| Formulation Format | GO-assisted CPP complex |
| Formulation Components | Graphene oxide + PF221 + siRNA |
| Size Nm | 133.00 |
| Zeta Mv | -25.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; U-87 MG-luc2; HeLa 705 |
| Animal Model | |
| Administration Route | cell culture |
| Output Type | gene knockdown |
| Output Value | Significant enhancement vs CPP alone |
| Output Units | |
| Output Notes | GO promotes endosomal escape and reduces toxicity |
| Toxicity Notes | Low cytotoxicity; GO mitigates CPP toxicity |
| Curation Notes |