Sequence: CYGRKKRRQRRR
| Experiment Id | EXP002339 |
|---|---|
| Paper | Mixed Nanosized Polymeric Micelles as Promoter of Doxorubicin and miRNA-34a Co-Delivery Triggered by |
| Peptide | TATp |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | component of micelle (5 mol%) |
| Rna Concentration | 100 nM (typical) |
| Mixing Ratio | PEG1k-PE : PEG2k-CLV-Dox : TAT-PEG1k-PE ≈ 90:5:5 |
| Formulation Format | polymeric mixed micelle |
| Formulation Components | PEG1k-PE + PEG2k-CLV-Dox + miRNA-34a-S-S-PE + TAT-PEG1k-PE |
| Size Nm | 15.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HT1080; MCF7; 3D HT1080 spheroids |
| Animal Model | |
| Administration Route | cell culture |
| Output Type | gene knockdown + cytotoxicity |
| Output Value | Bcl2 ↓65%, survivin ↓55%, notch1 ↓45%; viability ↓ to ~40% |
| Output Units | |
| Output Notes | Dual MMP2/GSH-triggered release; Tat enhances uptake and escape |
| Toxicity Notes | Low off-target toxicity; serum-compatible |
| Curation Notes |