Sequence: RRRRRRRR
| Experiment Id | EXP002346 |
|---|---|
| Paper | Coated microneedles mediated intradermal delivery of octaarginine/BRAF siRNA nanocomplexes for anti- |
| Peptide | R8 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | N/P ratio 10:1 |
| Rna Concentration | 100 nM |
| Mixing Ratio | N/P = 10 |
| Formulation Format | CPP/siRNA nanocomplex |
| Formulation Components | R8 + siBraf |
| Size Nm | 353.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A375 melanoma cells |
| Animal Model | |
| Administration Route | cell culture |
| Output Type | gene knockdown + proliferation inhibition |
| Output Value | BRAF mRNA ↓ ~88%; protein ↓ significantly |
| Output Units | |
| Output Notes | Comparable efficacy to PEI; low cytotoxicity |
| Toxicity Notes | Low toxicity compared to PEI |
| Curation Notes |