Sequence: RRRRRRRR
| Experiment Id | EXP002347 |
|---|---|
| Paper | Coated microneedles mediated intradermal delivery of octaarginine/BRAF siRNA nanocomplexes for anti- |
| Peptide | R8 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 16.5 μg siRNA per MN patch |
| Rna Concentration | 16.5 μg |
| Mixing Ratio | N/P = 10 |
| Formulation Format | Microneedle-coated CPP/siRNA nanocomplex |
| Formulation Components | R8/siBraf coated stainless-steel microneedles |
| Size Nm | 353.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | A375 melanoma-bearing Balb/c nude mice |
| Administration Route | intradermal (microneedles) |
| Output Type | tumor inhibition + gene silencing |
| Output Value | Tumor inhibition rate ~77%; BRAF mRNA ↓ ~31% |
| Output Units | |
| Output Notes | Reduced proliferation, increased apoptosis (PCNA/TUNEL) |
| Toxicity Notes | No significant body-weight loss |
| Curation Notes |