Sequence: YGRKKRRQRRR
| Experiment Id | EXP002350 |
|---|---|
| Paper | Novel miR-122 delivery system based on MS2 virus like particle surface displaying cell-penetrating p |
| Peptide | HIV-Tat (47–57) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 8 or 40 µg/mL (VLP concentration) |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | MS2 virus-like particle (VLP) displaying CPP |
| Formulation Components | 2MS-TAT-miR122 VLP (miR-122 packaged inside MS2 VLP; TAT displayed on surface) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Hep3B; HepG2; Huh7; HeLa |
| Animal Model | |
| Administration Route | cell culture |
| Output Type | miRNA functional effect (proliferation/migration/invasion; target protein suppression) |
| Output Value | Inhibited proliferation, migration, invasion; induced apoptosis; cyclin G1 & Igf1r downregulated |
| Output Units | |
| Output Notes | Mature miR-122 increased by RT-qPCR; downstream targets suppressed by WB; effects strongest in Hep3B |
| Toxicity Notes | CCK-8: viability >90% at 40 µg/mL; IC50 >100 µg/mL |
| Curation Notes |