Sequence: AGYLLGK INLKALAALAKKIL-amide
dsDNA transcription factor decoy
| Experiment Id | EXP002357 |
|---|---|
| Paper | Cellular delivery of a double-stranded oligonucleotide NFκB decoy by hybridization to complementary |
| Peptide | Transportan-10 (TP10) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP–PNA–DNA hybrid complex |
| Formulation Components | Transportan-10 (TP10) + PNA + NFκB decoy dsDNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Rat Rinm5F insulinoma cells |
| Animal Model | |
| Administration Route | cell culture incubation |
| Output Type | transcriptional inhibition |
| Output Value | Reduced NFκB DNA binding and IL-6 mRNA expression after IL-1β stimulation |
| Output Units | |
| Output Notes | Functional delivery requires CPP–PNA; decoy alone ineffective even at long incubation |
| Toxicity Notes | No cytotoxicity reported at 1 µM |
| Curation Notes |