Multistage pH-responsive codelivery liposomal platform for synergistic cancer therapy (2022)
DPRP (dual pH-responsive peptide)
Sequence: Ac-CGehGehGehGehGG-benzoic imine-rrrrrrrrGC
| Experiment Id | EXP002358 |
|---|---|
| Paper | Multistage pH-responsive codelivery liposomal platform for synergistic cancer therapy |
| Peptide | DPRP (dual pH-responsive peptide) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | pH-responsive liposome (DPRP-modified) |
| Formulation Components | DOPE/CHEMS/Chol + DSPE-PEG2000-DPRP + SA-R8 or SA-H8 + siRNA ± DTX |
| Size Nm | 180.00 |
| Zeta Mv | 35.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 |
| Animal Model | |
| Administration Route | cell culture |
| Output Type | gene silencing + apoptosis |
| Output Value | PLK-1 mRNA/protein downregulation; apoptosis up to ~58% (pH 6.5) |
| Output Units | |
| Output Notes | pH-triggered CPP activation, endosomal escape and cytosolic release shown |
| Toxicity Notes | Low toxicity to control cells |
| Curation Notes |