Sequence: FAEKFKEAVKDYFAKFWDGSGCGNKRTR
| Experiment Id | EXP002363 |
|---|---|
| Paper | Lipoprotein-biomimetic nanostructure enables tumor-targeted penetration delivery for enhanced photo- |
| Peptide | tLyP-1-D4F |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 nM (siRNA) |
| Mixing Ratio | |
| Formulation Format | HDL-mimetic nanoparticle co-loading ICG + Chol-siRNA |
| Formulation Components | ptHDL/siHIF-ICG: PC + tLyP-1-D4F + ICG + Chol-siHIF |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 (glioma); bEnd.3 (BBB model); U87 tumor spheroids |
| Animal Model | |
| Administration Route | cell culture (± BBB transwell model; ± 808 nm laser) |
| Output Type | gene knockdown + photo-gene cytotoxicity |
| Output Value | HIF-1α and CD31 downregulated; viability inhibition up to ~95.7% with laser |
| Output Units | |
| Output Notes | Tumor-penetrating HDL-mimic built from fusion peptide; improved BBB crossing, tumor homing/penetration via NRP-1 |
| Toxicity Notes | Negligible dark cytotoxicity in bEnd.3 |
| Curation Notes |