Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery (2014)
Sequence: cyclo-(WRWRWRWRWR)
| Experiment Id | EXP002367 |
|---|---|
| Paper | Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery |
| Peptide | WR5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 25 µM |
| Rna Concentration | 200 nM |
| Mixing Ratio | |
| Formulation Format | peptide or peptide-capped gold nanoparticle |
| Formulation Components | [WR]5 ± AuNPs + siRNA (non-covalent complex) |
| Size Nm | 200.00 |
| Zeta Mv | 56.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa (human cervical adenocarcinoma) |
| Animal Model | |
| Administration Route | cell culture transfection |
| Output Type | siRNA uptake (no functional knockdown focus) |
| Output Value | 2-fold (WR5) and 3.8-fold (WR5-AuNPs) increased uptake vs siRNA alone |
| Output Units | |
| Output Notes | Flow cytometry and fluorescence microscopy; uptake not blocked by endocytosis inhibitors |
| Toxicity Notes | Low cytotoxicity vs Lipofectamine, TAT, CR7 |
| Curation Notes |