Sequence: GRKKRRQRRR
| Experiment Id | EXP002369 |
|---|---|
| Paper | Blockade of HIF-1α and STAT3 by hyaluronate-conjugated TAT-chitosan-SPION nanoparticles loaded with |
| Peptide | HIV-Tat |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | polymer-coated magnetic nanoparticle |
| Formulation Components | SPION-TMC-ChT-TAT-HA + siRNA |
| Size Nm | 118.00 |
| Zeta Mv | 20.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | 4T1 (breast cancer); CT26 (colon cancer) |
| Animal Model | |
| Administration Route | cell culture transfection |
| Output Type | gene knockdown; anti-proliferative and pro-apoptotic effects |
| Output Value | Significant reduction of HIF-1α and STAT3 mRNA/protein; reduced proliferation and migration |
| Output Units | |
| Output Notes | qPCR, Western blot, BrdU, migration assays |
| Toxicity Notes | Low toxicity for blank nanoparticles |
| Curation Notes |