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EXP002371

Paper

Antisense peptide nucleic acids against ftsZ and efaA genes inhibit growth and biofilm formation of Enterococcus faecalis (2020)

Peptide

(RXR)4 carrier peptide

Sequence: RXRRXRRXRRXRR

RNA

PNA (antisense oligonucleotide)

All experiment fields

Experiment Id EXP002371
Paper Antisense peptide nucleic acids against ftsZ and efaA genes inhibit growth and biofilm formation of
Peptide (RXR)4 carrier peptide
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio
Formulation Format CPP–PNA conjugate
Formulation Components (RXR)4–efaA PNA
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Enterococcus faecalis ATCC 29212 (bacteria)
Animal Model
Administration Route direct incubation
Output Type gene knockdown and biofilm inhibition
Output Value ~95–99% reduction in efaA expression; significant biofilm reduction
Output Units
Output Notes qRT-PCR and crystal violet biofilm assays
Toxicity Notes No toxicity in MCF7 cells (MTT assay)
Curation Notes