Targeting Listeria monocytogenes rpoA and rpoD genes using peptide nucleic acids (2013)
Sequence: KFFKFFKFFK-cgatcattcaaa
PNA (antisense oligonucleotide)
| Experiment Id | EXP002380 |
|---|---|
| Paper | Targeting Listeria monocytogenes rpoA and rpoD genes using peptide nucleic acids |
| Peptide | anti-rpoA (KFF)3K-PNA |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP–PNA conjugate |
| Formulation Components | (KFF)3K–PNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Listeria monocytogenes culture; J774A.1 macrophages |
| Animal Model | |
| Administration Route | direct incubation |
| Output Type | bacterial growth inhibition / intracellular clearance |
| Output Value | Dose-dependent CFU reduction; significant intracellular bacterial reduction |
| Output Units | |
| Output Notes | Pure culture, macrophage infection assays |
| Toxicity Notes | No host cell toxicity reported |
| Curation Notes |