Targeting Listeria monocytogenes rpoA and rpoD genes using peptide nucleic acids (2013)
Sequence: KFFKFFKFFK-cgatcattcaaa
PNA (antisense oligonucleotide)
| Experiment Id | EXP002381 |
|---|---|
| Paper | Targeting Listeria monocytogenes rpoA and rpoD genes using peptide nucleic acids |
| Peptide | anti-rpoA (KFF)3K-PNA |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP–PNA conjugate |
| Formulation Components | (KFF)3K–PNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Caenorhabditis elegans infected with L. monocytogenes |
| Administration Route | direct exposure |
| Output Type | bacterial load reduction / survival |
| Output Value | Anti-rpoA shows 37–72% bacterial reduction; anti-rpoD weaker |
| Output Units | |
| Output Notes | Whole-animal infection model |
| Toxicity Notes | No worm toxicity observed |
| Curation Notes |