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EXP002385

Paper

Bacterial outer membrane vesicle nanorobot (2024)

Peptide

CPP (ClyA-CPP fusion)

Sequence: CGKRK

RNA

siRNA

All experiment fields

Experiment Id EXP002385
Paper Bacterial outer membrane vesicle nanorobot
Peptide CPP (ClyA-CPP fusion)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio
Formulation Format OMV-based enzyme-powered nanorobot
Formulation Components CPP-OMV + urease + siRNA
Size Nm 115.00
Zeta Mv 20.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells MBT-2 bladder cancer cells
Animal Model
Administration Route cell culture incubation
Output Type gene knockdown; apoptosis
Output Value Strong survivin mRNA/protein reduction; increased apoptosis
Output Units
Output Notes qPCR, Western blot, Annexin V/PI; propulsion-enhanced uptake
Toxicity Notes Low toxicity at working concentrations
Curation Notes