Bioengineered Extracellular Membranous Nanovesicles for Efficient Small-Interfering RNA Delivery (2016)
Sequence: RGD
| Experiment Id | EXP002387 |
|---|---|
| Paper | Bioengineered Extracellular Membranous Nanovesicles for Efficient Small-Interfering RNA Delivery |
| Peptide | RGD |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | engineered exosome / PMNV |
| Formulation Components | RGD-modified exosome or PMNV + siRNA |
| Size Nm | 100.00 |
| Zeta Mv | 12.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa, hADSCs, hNSCs, hiPSCs |
| Animal Model | |
| Administration Route | cell culture incubation |
| Output Type | gene knockdown / differentiation |
| Output Value | Efficient knockdown of GFP/GAPDH/REST/GNAS; promoted differentiation |
| Output Units | |
| Output Notes | qPCR, FACS, immunostaining |
| Toxicity Notes | Negligible cytotoxicity |
| Curation Notes |