Protein and siRNA delivery by transportan and transportan 10 into colorectal cancer cell lines (2014)
Sequence: GWTLNSAGYLLGKINLKALAALAKKIL-amide
| Experiment Id | EXP002392 |
|---|---|
| Paper | Protein and siRNA delivery by transportan and transportan 10 into colorectal cancer cell lines |
| Peptide | TP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.5–5 µM |
| Rna Concentration | 10–50 nM |
| Mixing Ratio | non-covalent; CPP 2 µM with siRNA |
| Formulation Format | CPP/siRNA non-covalent complex |
| Formulation Components | transportan or TP10 derivative + siRNA in culture medium |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HT29 (early-stage CRC); HCT116 (metastatic CRC) |
| Animal Model | |
| Administration Route | cell culture incubation |
| Output Type | gene knockdown (qPCR) |
| Output Value | SASH1 mRNA reduction (significant at 50 nM siRNA) |
| Output Units | |
| Output Notes | Functional siRNA delivery; streptavidin-FITC uptake shown. Endocytosis-independent delivery demonstrated only for TP-biot1 and TP10-biot1 using brefeldin A. |
| Toxicity Notes | No significant cytotoxicity at ≤5 µM for any peptide. |
| Curation Notes |