Inhibition of SARS-CoV-2 in Vero cell cultures by peptide-conjugated morpholino oligomers (2021)
Sequence: (Arg–Ahx–Arg)4
PMO (antisense morpholino oligomer)
| Experiment Id | EXP002402 |
|---|---|
| Paper | Inhibition of SARS-CoV-2 in Vero cell cultures by peptide-conjugated morpholino oligomers |
| Peptide | (RXR)4 CPP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 4, 8, or 16 µM PPMO |
| Rna Concentration | PMO component within PPMO (same molar concentration) |
| Mixing Ratio | covalent CPP–PMO conjugate |
| Formulation Format | peptide–PMO conjugate (PPMO) |
| Formulation Components | (RXR)4 CPP covalently linked to antisense PMO |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Vero-E6 cells |
| Animal Model | |
| Administration Route | 5 h pretreatment of cells prior to viral infection |
| Output Type | viral replication inhibition |
| Output Value | 4–6 log10 reduction in viral titers for active PPMO |
| Output Units | |
| Output Notes | qRT–PCR and TCID50 assays show strong suppression for 5′END and TRS PPMO; AUG less effective; NC inactive. |
| Toxicity Notes | <5% cytotoxicity at tested doses (ATP assay) |
| Curation Notes |