CAP (cartilage-targeting peptide)
Sequence: DWRVIIPPRPSA
| Experiment Id | EXP002412 |
|---|---|
| Paper | Co-Polymer Carrier with Dual Advantages of Cartilage-Penetrating and Targeting Improves Delivery and |
| Peptide | CAP (cartilage-targeting peptide) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ~30 CAP molecules per PVAm chain (≈2% grafting ratio) |
| Rna Concentration | 20 nM r-miR-140 (in vitro) |
| Mixing Ratio | CAP-PVAm-PLGA : r-miR-140 = 1 (w/w) |
| Formulation Format | polymeric nanoparticles (CPP-NPs) |
| Formulation Components | CAP-PVAm-PLGA copolymer + r-miR-140 |
| Size Nm | 96.00 |
| Zeta Mv | 8.80 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Primary mouse chondrocytes; hMSC; MC3T3-E1 |
| Animal Model | |
| Administration Route | cell culture transfection |
| Output Type | gene regulation, chondroprotection |
| Output Value | Restored miR-140 levels; ↓IL-6, ↓MMP-13, ↓ADAMTS-5; ↑Col2a1 |
| Output Units | |
| Output Notes | Efficient uptake, lysosomal escape demonstrated by Lysotracker colocalization reduction |
| Toxicity Notes | Low cytotoxicity and hemolysis |
| Curation Notes |